01728nas a2200157   4500000000100000008004100001100001500042700001300057700002200070700001400092245013700106856007000243490000700313520123600320022001401556       2019                            d1 aNovianto E1 aManalu P1 aEffendi Wiryadi B1 aMenaldi S00aMycobacterium leprae deoxyribonucleic acid positivity on skin lesion of untreated leprosy patients and its route to the skin surface  uhttps://www.pagepress.org/journals/index.php/dr/article/view/80240 v113 a<p>The route of transmission of leprosy is still unclear. Multiple opinions exist regarding the environmental dissemination of <em>Mycobacterium leprae</em> (<em>M. leprae</em>). This research aims to identify the positive proportion of <em>M. leprae</em> deoxyribonucleic acid (DNA) on skin lesion surface of untreated multibacillary leprosy patients by polymerase chain reaction (PCR), and its association with the examination result of acid-fast bacilli (AFB) in epidermis, pilosebaceous unit, and sweat glands. The <em>M. leprae</em> DNA sample was taken from smears of the skin lesion surface by using PCR and the skin biopsy test was done to detect AFB in epidermis, pilosebaceous unit, and sweat glands. From 28 subjects, there was 82.1% positive proportion of <em>M. leprae</em> DNA on the skin lesion surface of untreated multibacillary leprosy patients, where all were found in the lepromatous leprosy and borderline leprosy type. The result of AFB was positive in epidermis (71.4%), pilosebaceous unit (46.4%) and sweat glands (53.6%). We found <em>M. leprae</em> by using PCR technique on skin lesion surface of untreated multibacillary leprosy patients, and its route which most likely was through the epidermis.</p>
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