01613nas a2200337   4500000000100000008004100001260001300042653001800055653001600073653001900089653001100108653001200119653002500131653003100156653003000187653002400217653003200241100001300273700001200286700001000298700001000308700001400318700001400332700001400346700001300360245005900373300001100432490000700443520081100450022001401261       1999                            d  c1999 Dec10aChaperonin 6010aDNA Primers10aDNA, Bacterial10aHumans10aleprosy10aMycobacterium leprae10aMycobacterium tuberculosis10apolymerase chain reaction10aRNA, Ribosomal, 16S10aSensitivity and Specificity1 aQinxue W1 aXinyu L1 aWei H1 aTao L1 aYaoping Y1 aJinping Z1 aXiuling C1 aGanyun Y00aA study on PCR for detecting infection with M. leprae.  a237-410 v143 a<p><b>OBJECTIVE: </b>So far, it has not been established a satisfactory method for early diagnosis and studying on epidemiology for leprosy, we want to develop a molecular biological method for solving this point.</p><p><b>MATERIALS AND METHODS: </b>Based on the M. leprae gene coding groEL, 65 kD and 16S rRNA, three polymerase chain reactions were developed by using Plikaytis', Woods' and Pattyn's procedures. It was optimized that the experimental parameters for each PCR, and a comparative study on practivity among three PCRs was also conducted for practical purpose.</p><p><b>RESULTS AND CONCLUSION: </b>For detecting infection with M. leprae, all of PCRs established by us were highly sensitive and specific, but for practical purpose, the Woods' PCR optimized by us ought to be chosen firstly.</p>  a1001-9294