01964nas a2200241   4500000000100000008004100001653001200042653002100054653002600075653002200101653001400123653002900137100001400166700001500180700001300195700001400208700001200222700001600234700001700250245008300267520135800350022001401708       2018                            d10aleprosy10aMonocyte subsets10aMarkers of activation10aLeprosy reactions10aMonocytes10aCo-stimulatory molecules1 aShibuya M1 aBergheme G1 aPassos S1 aQueiroz I1 aRêgo J1 aCarvalho LP1 aMachado PR L00aEvaluation of monocyte subsets and markers of activation in leprosy reactions.3 a<p>Understanding host immune pathways associated with tissue damage during reactions are of upmost importance to the development of immune intervention strategies. The participation of monocytes in leprosy reactions was evaluated by determining the frequency of monocyte subsets and the degree of cellular activation through the expression of MHCII and the co-stimulatory molecules CD40, CD80, CD86. Leprosy subjects with or without reactions were included in this cross-sectional study. Peripheral blood mononuclear cell were isolated and stained ex vivo to determine monocyte subsets and the degree of cellular activation by flow cytometry. Intermediate monocytes were increased in leprosy patients with reactions when compared to patients without reactions. Although no difference was detected in the frequency of monocyte subsets between type 1 and 2 reactions, the expression of CD80 was increased in monocytes from patients with type 1 reactions and CD40 was higher in paucibacillary subjects presenting type 1 reactions. Moreover, CD86 and MHC II expression were higher in intermediate monocytes when compared to the other subsets in leprosy reaction types 1 and 2. Intermediate monocyte activation with CD86 and MHCII expression is involved with both type 1 and 2 reactions, whereas CD80 and CD40 expression is related to type 1 reactions.</p>
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