02375nas a2200277   4500000000100000008004100001653001000042653001000052653001000062653001200072653002300084653001500107653001000122100001400132700001000146700001200156700001200168700001200180700001000192700001300202700001300215700001100228700001300239245013100252520171400383       2018                            d10aBregs10aFoxP310aIL-1010aleprosy10aRegulatory B cells10aTeff cells10aTregs1 aTarique M1 aNaz H1 aKurra S1 aNaqvi R1 aSaini C1 aRai R1 aSuhail M1 aKhanna N1 aRao DN1 aSharma A00aIL-10-producing regulatory B cells transformed CD4+CD25- into Tregs and enhanced regulatory T cells function in human leprosy.3 a<p>Regulatory B cells are known to exhibit their regulatory functions through Interleukin 10 (IL-10) cytokine which suppresses inflammation. There are only a few studies explaining the phenotype and functioning of these cells in contribution to host immunity in leprosy. Here, we evaluated the role of interleukin-10 producing regulatory B-cells (Bregs) in the pathogenesis of leprosy and assessed their immunoregulatory effects on Tregs and effector T cells. We found an increased frequency of Bregs and increased expression of their immune modulatory molecules (IL-10, FoxP3 and PDL-1) in leprosy patients. The potential immunoregulatory mechanism of Bregs was also investigated using MACS sorted Teff (CD4+CD25-) and Treg (CD4+CD25+) cells were co-cultured with Bregs to elucidate the effects of Bregs on effector T and regulatory T-cells. Cell co-culture results showed that purified Bregs cells from leprosy patients convert the CD4+CD25- cells into CD4+CD25+ cells. Cell co-culture experments also demonstrated that leprosy derived IL-10 producing Bregs enhance the FoxP3 and PD-1 expression in Tregs and enhanced Tregs activity. Blocking of IL-10 receptor confirmed that IL-10 producing Breg has immunomodulatory effect on Tregs and effector T cells as effector T cells and are not converted into Tregs and enhanced expression of FoxP3 and PD-1 was not observed on Tregs. Collectively, these findings demonstrate that IL-10-producing Breg cells play an important mechanism in controlling the immunopathogenesis of leprosy and have an immunomodulatory effect on Tregs and effector T cells. Our findings may pave way for novel targets of IL-10 producing Bregs for immunotherapy in leprosy patients.</p>