02452nas a2200409   4500000000100000008004100001260001300042653001000055653000900065653002900074653001400103653001100117653001400128653001100142653001100153653003800164653002300202653001200225653000900237653001600246653001900262653001500281653001900296653001600315653003200331100001200363700001300375700001700388700001300405700001200418700001400430245008900444300001100533490000800544520147600552022001402028       2002                            d  c2002 Nov10aAdult10aAged10aAnti-Inflammatory Agents10aCytokines10aDermis10aEpidermis10aFemale10aHumans10aIntercellular Adhesion Molecule-110aLeprostatic Agents10aleprosy10aMale10aMiddle Aged10aPentoxifylline10aPrednisone10aRNA, Messenger10aThalidomide10aTumor Necrosis Factor-alpha1 aTeles R1 aMoraes M1 aGeraldo NT R1 aSalles A1 aSarno E1 aSampaio E00aDifferential TNFalpha mRNA regulation detected in the epidermis of leprosy patients.  a355-620 v2943 a<p>The epidermis is an important site of the immunoinflammatory response in the skin. In the present study, the expression of cytokine and ICAM-1 (intercellular adhesion molecule-1) genes was evaluated by RT-PCR in the epidermis isolated from biopsies from 25 reactional leprosy patients. TNFalpha and IL-6 mRNAs were detected in all individuals during the reactional state (reversal reaction or erythema nodosum leprosum), IL-8 message was detected in 66.6% and 62.5% of the patients, IL-12 mRNA was present in 91.6% and 62.5% and ICAM-1 in 100% and 71.4%, respectively. In addition, when skin biopsies were obtained from the same patients before and during the reactional episode, an enhancement in cytokine mRNA, but not in ICAM-1 mRNA, was observed. Seven patients were also evaluated at the onset of reaction and during antiinflammatory treatment. In contrast to a preferential decrease in the TNFalpha gene detected in the dermis, during the treatment phase, persistent/enhanced TNFalpha mRNA expression was detected in the epidermis in six out of the seven patients assessed. This peculiar pattern of expression might reflect a differential impact that in vivo antiinflammatory therapy has on the epidermis. The present findings indicate that the epidermis plays an important role in the local inflammatory response in leprosy and that the profile of response detected in the epidermis during the reactions may be regulated differently from that in the dermis.</p>  a0340-3696