02155nas a2200445   4500000000100000008004100001260001600042653001500058653001000073653000900083653001200092653001100104653001900115653001100134653001900145653001200164653000900176653001600185653002600201653003000227653003200257100001400289700001300303700001900316700001300335700001600348700001400364700001300378700001200391700001800403700001600421700001400437700001200451245009900463300001200562490000800574050001600582520109700598022001401695       2002                            d  c2002 Dec 0110aAdolescent10aAdult10aAged10aAlleles10aFemale10aGene Frequency10aHumans10aInterleukin-1010aleprosy10aMale10aMiddle Aged10aPolymorphism, Genetic10aPromoter Regions, Genetic10aTumor Necrosis Factor-alpha1 aSantos AR1 aSuffys P1 aVanderborght P1 aMoraes M1 aVieira LM M1 aCabello P1 aBakker A1 aMatos H1 aHuizinga TW J1 aOttenhoff T1 aSampaio E1 aSarno E00aRole of tumor necrosis factor-alpha and interleukin-10 promoter gene polymorphisms in leprosy.  a1687-910 v186  aSANTOS 20023 a<p>Single-nucleotide polymorphisms within the genes coding for tumor necrosis factor (TNF)-alpha and interleukin (IL)-10 have been associated with several infectious diseases. To determine whether such polymorphisms are associated with leprosy, genotyping was performed at the -308 and -238 positions of the promoter of the TNF-alpha gene in 210 and 191 patients with multibacillary (MB) leprosy, respectively; 90 and 79 patients with paucibacillary (PB) leprosy; and 92 control subjects. For the -592 and -819 positions within the promoter of the IL-10 gene, 143 patients with MB leprosy, 79 patients with PB leprosy, and 62 control subjects were included in the analysis. TNF2 allele frequency was significantly higher among control subjects than among all patients with leprosy or in the MB group (P<.05 and P<.01). For the IL-10 gene, the frequency of the homozygous -819TT genotype was significantly higher among patients than among control subjects. These data indicate that a relationship exists between TNF-alpha and IL-10 promoter polymorphisms and the development of PB leprosy.</p>  a0022-1899