02515nas a2200457   4500000000100000008004100001260001300042653001200055653002600067653002300093653002300116653001800139653001600157653001600173653001400189653001100203653001700214653001200231653000900243653002400252653002500276653001700301653003700318653001800355100001600373700001200389700001600401700001500417700001200432700001100444700001200455700001100467700001100478700001100489245014300500856004100643300001100684490000700695520134100702022001402043       2002                            d  c2002 Sep10aAnimals10aAntibodies, Bacterial10aBacterial Proteins10aBacterial Vaccines10aChaperonin 6010aChaperonins10aCpG Islands10aCytokines10aFemale10aImmunization10aleprosy10aMice10aMice, Inbred BALB C10aMycobacterium leprae10aNitric Oxide10aSpecific Pathogen-Free Organisms10aVaccines, DNA1 aNomaguchi H1 aMukai T1 aTakeshita F1 aMatsuoka M1 aMaeda Y1 aAye TM1 aJahan N1 aYogi Y1 aEndo M1 aSato Y00aEffect of hsp65 DNA vaccination carrying immunostimulatory DNA sequences (CpG motifs) against Mycobacterium leprae multiplication in mice.  uhttp://ila.ilsl.br/pdfs/v70n3a03.pdf  a182-900 v703 a<p>A DNA expressing hsp65 of Mycobacterium leprae (pACB/hsp65) was constructed by using a vector containing immunostimulatory DNA sequences (pACB). At 12 weeks post-immunization, spleen cells from BALB/cA mice immunized with pACB/hsp65, produced a significantly higher amount of IFN-gamma than mice immunized with pACB in the absence of any in vitro stimulation, and further enhanced its production upon secondary in vitro stimulation with M. leprae lysate and hsp65. On the other hand, while production of IL-12 was observed in mice immunized with pACB/hsp65 12 weeks before, the cytokine production was inhibited by in vitro secondary stimulation with M. leprae or hsp65. At 18 weeks post-immunization, the production of both IFN-gamma and IL-12 was apparently down-regulated, but that of IL-10 was up-regulated. IL-10 seemed to suppress the IFN-gamma and IL-12 productions, because their production was recovered by neutralization of IL-10 with anti-IL-10 mAb. Furthermore, when the efficiency of pACB/hsp65 as a vaccine against M. leprae was evaluated in vivo, the mice immunized with pACB/hsp65 suppressed the multiplication of subsequently challenged M. leprae. These results suggest that a DNA containing M. leprae-derived hsp65 and immunostimulatory sequences might be a potent vaccine candidate against M. leprae infection.</p>  a0148-916X