01162nas a2200145   4500000000100000008004100001100001400042700001600056700001500072245007700087856005500164490000700219520077600226022001401002       2017                            d1 aYadav A R1 aMohanty K K1 aSengupta U00aICRC Bacillus a Vaccine Candidate Strain (C-44) Is Coated with Human IgG  uhttp://file.scirp.org/pdf/OJI_2017090713513171.pdf0 v073 a<p>Indian Cancer Research Centre (ICRC) bacillus strain (C-44), a candidate vaccine against leprosy is cultured in vitro in Dubos medium enriched with amino acids and human serum. The study was conducted to find out whether ICRC bacilli obtained from these cultures are coated with anti mycobacterial antibody. Anti-ICRC antibody raised by intradermal inoculation of sonicated ICRC bacilli in rabbits reacted with both human immunoglobulin G (IgG) and antigens of ICRC. Further, ICRC bacilli could also be fluoresced directly with FITC labelled anti human IgG. Positive fluorescence of ICRC could be abolished by digestion of human IgG with trypsin and carbon tetrachloride (CCL4). It is concluded that ICRC bacilli present in the vaccine are coated with human IgG.</p>
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