01304nas a2200205   4500000000100000008004100001653001400042653001700056653001200073653001400085100001400099700001400113700001900127245007100146856010100217300001200318490000800330520074600338022001401084       2017                            d10aZebrafish10aMycobacteria10aleprosy10aGranuloma1 aMadigan C1 aCameron J1 aRamakrishnan L00aA Zebrafish Model of Mycobacterium leprae Granulomatous Infection.  uhttps://academic.oup.com/jid/article/216/6/776/3978667/A-Zebrafish-Model-of-Mycobacterium-leprae  a776-7790 v2163 a<p>Understanding the pathogenesis of leprosy granulomas has been hindered by a paucity of tractable experimental animal models. Mycobacterium leprae, which causes leprosy, grows optimally at approximately 30°C, so we sought to model granulomatous disease in the ectothermic zebrafish. We found that noncaseating granulomas develop rapidly and eventually eradicate infection. rag1 mutant zebrafish, which lack lymphocytes, also form noncaseating granulomas with similar kinetics, but these control infection more slowly. Our findings establish the zebrafish as a facile, genetically tractable model for leprosy and reveal the interplay between innate and adaptive immune determinants mediating leprosy granuloma formation and function.</p>
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