02861nas a2200385 4500000000100000008004100001653002100042653001000063653001600073653003200089653000900121653001100130653001000141653001100151653002700162653003000189653002800219653001800247653001100265653001300276653002600289653002100315653001000336100001200346700001500358700001200373700001600385700001300401245011700414856008800531300000800619490000700627520182700634022001402461 2016 d10aSex Distribution10aNepal10aMiddle Aged10aMicrobial Sensitivity Tests10aMale10aHumans10aFungi10aFemale10aEye Infections, Fungal10aEye Infections, Bacterial10aCross-Sectional Studies10aCorneal Ulcer10aCornea10aBacteria10aAnti-Bacterial Agents10aAge Distribution10aAdult1 aSuwal S1 aBhandari D1 aThapa P1 aShrestha MK1 aAmatya J00aMicrobiological profile of corneal ulcer cases diagnosed in a tertiary care ophthalmological institute in Nepal. uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5129215/pdf/12886_2016_Article_388.pdf a2090 v163 a
BACKGROUND: Corneal ulcer, a major cause of monocular blindness in developing countries has consistently been listed as the major cause of blindness and visual disability in many of the developing nations in Asia, Africa and the Middle East, ranking second only to cataract. This study was carried out to determine the microbiological profile of corneal ulcer cases diagnosed among patients visiting Tilganga Institute of Ophthalmology (TIO), Nepal.
METHODS: A total of 101 corneal scrapping samples were tested for routine culture and antibiotic susceptibility at the pathology department of TIO Nepal from April to October 2014. Microorganisms were identified by using standard microbiological procedures following the manual of American Society for Microbiology (ASM) and their antibiotic susceptibility test, performed by Kirby-Bauer disc diffusion method in conformity with the CLSI guideline.
RESULTS: Out of 101 samples analyzed, 44.6% (45/101) showed positive growth with bacterial isolates i.e., 56% (25/45), more prevalent than fungus i.e., 44% (20/45). Among bacteria Streptococcus pneumoniae (31.1%, Nā=ā14) was isolated in highest number whereas Fusarium (13.4%, Nā=ā6) was the most common fungus species. Pseudomonas aeruginosa was the only Gram negative bacteria isolated from corneal ulcer cases. All bacterial isolates were found to be susceptible to the quinolone group of antibiotics (moxifloxacin followed by ofloxacin and ciprofloxacin).
CONCLUSIONS: These findings showcase the current trend in the microbiological etiology of corneal ulcer in Nepal, which have important public health implications for the treatment as well as prevention of corneal ulceration in the developing world.
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