02233nas a2200241   4500000000100000008004100001653002500042653002500067653001200092653003100104653002500135100001000160700001300170700001100183700001300194700001600207245011300223856003100336300001400367490000600381520159100387022001301978       2017                            d10aPutative drug target10aMycobacterium leprae10aleprosy10aInositol metabolic pathway10aHypothetical protein1 aRaj U1 aPathak S1 aAier I1 aGrupta S1 aVaradwaj PK00aIn silico finding of the putative drug targets from hypothetical set of proteins for Mycobacterium leprae TN  uhttp://tinyurl.com/mvyje35  a2100-21210 v83 a<p>Leprosy is a chronic granulomatous disease caused by acid-fast bacilli <em>Mycobacterium leprae</em>. It is most prevalent in tropical countries and poses a threat to over 122 countries across the globe. In 2012, 232857 new cases of leprosy were registered in the world. World Health Organization (WHO), recommended the Multi Drug Therapy (MDT) for treatment of leprosy, but the minimum duration of treatment ranges from 6-12 months. Therefore, the need of a new putative target for treatment of leprosy, which is effective in leprosy and reduces the duration of treatment. The Proteome information available suggests that among the 1603 proteins in <em>M. leprae</em> TN, a staggering 27% or more remains classified as hypothetical uncharacterized set of proteins. In this present work we, assign the probable functions of hypothetical set of proteins present in <em>M. leprae </em>to explore their plausible role as new putative drug targets. Out of 442,177 hypothetical protein sequences had GO term. Of these, 43 sequences had disease ontology (DO) term, 59 sequences had human phenotype (HP) term and out of these 43 sequences, 16 sequences were found to contain only DO term, while 27 sequences had both HP and DO term. Out of 177, 15 sequences were found to be associated with 39 KEGG reference pathway pathways. Out of 39 pathways, inositol phosphate metabolism, fatty acid degradation, ethylbenzene degradation, sulfur relay system and limonene &amp; pinene degradation were common metabolic pathway, which might be used as putative drug target for <em>M. leprae.</em></p>
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