01867nas a2200301   4500000000100000008004100001100001500042700001400057700001500071700001700086700002100103700001900124700001200143700001300155700001700168700001700185700001700202700001500219700001400234700001400248700001300262245006100275856007500336300001000411490000600421520112400427022001401551       2017                            d1 aFedrizzi T1 aMeehan CJ1 aGrottola A1 aGiacobazzi E1 aFregni Serpini G1 aTagliazucchi S1 aFabio A1 aBettua C1 aBertorelli R1 aDe Sanctis V1 aRumpianesi F1 aPecorari M1 aJousson O1 aTortoli E1 aSegata N00aGenomic characterization of Nontuberculous Mycobacteria.  uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5366915/pdf/srep45258.pdf  a452580 v73 a<p>Mycobacterium tuberculosis and Mycobacterium leprae have remained, for many years, the primary species of the genus Mycobacterium of clinical and microbiological interest. The other members of the genus, referred to as nontuberculous mycobacteria (NTM), have long been underinvestigated. In the last decades, however, the number of reports linking various NTM species with human diseases has steadily increased and treatment difficulties have emerged. Despite the availability of whole genome sequencing technologies, limited effort has been devoted to the genetic characterization of NTM species. As a consequence, the taxonomic and phylogenetic structure of the genus remains unsettled and genomic information is lacking to support the identification of these organisms in a clinical setting. In this work, we widen the knowledge of NTMs by reconstructing and analyzing the genomes of 41 previously uncharacterized NTM species. We provide the first comprehensive characterization of the genomic diversity of NTMs and open new venues for the clinical identification of opportunistic pathogens from this genus.</p>
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