02153nas a2200265   4500000000100000008004100001653002600042653001200068653000900080653001700089653001400106100001200120700001400132700001200146700001400158700001200172700001200184700001200196700001600208700001700224700001500241245011500256520150200371022001401873       2017                            d10aHeterozygous genotype10aleprosy10aNOS210aPolymorphism10aReactions1 aDubey A1 aBiswas SK1 aSinha E1 aChakma JK1 aKamal R1 aArora M1 aSagar H1 aNatarajan M1 aBhagyawant S1 aMohanty KK00aAssociation of Nitric Oxide Synthase2 gene polymorphisms with leprosy reactions in northern Indian population.3 a<p>The pathogen Mycobacterium leprae causes leprosy that affects mainly skin and nerves. Polymorphisms of certain genes are substantiated to be associated with the susceptibility/resistance to leprosy. The present investigation addressed the association of Nitric Oxide Synthase2 gene polymorphisms and leprosy in a population from northern part of India. A total of 323 leprosy cases and 288 healthy controls were genotyped for four NOS2 promoter variants (rs1800482, rs2779249, rs8078340 and rs2301369) using FRET technology in Real Time PCR. None of these SNPs in promoter sites was associated with susceptibility/resistance to leprosy. NOS2 rs1800482 was found to be monomorphic with GG genotype. However, NOS2-1026T allele was observed to be in higher frequency with leprosy cases (BL and LL) who were not suffering from any reactional episodes compared to cases with ENL reaction {OR=0.30, 95% CI (0.10-0.86), p=0.024}. NOS2-1026GT genotype was more prevalent in cases without reaction (BT, BB and BL) compared to RR reactional patients {OR=0.38, 95% CI (0.17-0.86), p=0.02}. Although haplotype analysis revealed that no haplotype was associated with leprosy susceptibility/resistance with statistical significance, GTG haplotype was noted to be more frequent in healthy controls. These SNPs are observed to be in linkage disequilibrium. Although, these SNPs are not likely to influence leprosy vulnerability, -1026G&gt;T SNP was indicated to have noteworthy role in leprosy reactions.</p>
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