02787nas a2200313   4500000000100000008004100001653002500042653001100067653001100078653001000089653002600099653001600125653000900141653002800150653002500178653000900203100001500212700001600227700001500243700001300258700001200271700001500283245007300298856005100371300001100422490000700433520201900440022001402459       2013                            d10aImmunohistochemistry10aHumans10aFemale10aAdult10aRetrospective Studies10aMiddle Aged10aMale10aLeprosy, Multibacillary10aLeprosy, lepromatous10aSkin1 aDa Costa D1 aEnokihara M1 aNonogaki S1 aMaeda SM1 aPorro A1 aTomimori J00aWade histoid leprosy: histological and immunohistochemical analysis.  uhttps://leprosyreview.org/article/84/3/17-6185  a176-850 v843 a<p>Histoid leprosy is a rare multibacillary form that presents with disseminated papule-nodular cutaneous lesions. To study the inflammatory infiltrate of the histoid form and to compare it with other lepromatous forms, we performed histological and immunohistochemical analysis on skin biopsies. Fifteen patients were included for histopathological analysis (10 histoid and five lepromatous) via the haematoxylin-eosin and Ziehl-Neelsen-Faraco stains. Thus, immunohistochemical techniques using immunoperoxidase assay were performed for: anti-BCG, anti-M. leprae, anti-CD8, anti-CD3, anti-CD20, anti-S100, anti-CD1a, anti-CD68 and antivimentin. Spindle cells were present in all histoid patients. A pseudocapsule was observed in half of both studied forms. A comparison using the Ziehl-Neelsen-Faraco stain to evaluate anti-BCG and anti-M.leprae showed no major differences. The CD3+ cells were more pronounced in the histoid form than the lepromatous form. There was greater immunoreactivity toward CD8+ cells in the histoid form, as well as the CD20+ cell count. A similar count of S100+ cells in the epidermis of both leprosy forms was observed. There was a slight increase of dendritic cells in the histoid patients in the superficial and deep dermis. For CD1a marker, we observed expression in the epidermis and superficial dermis in both forms. A diffuse and intense infiltrate of CD68+ cells was also observed in the histoid and lepromatous forms. The high positivity for vimentin did not allow for a positive cell count. We concluded that the activation of both the cellular and humoral response is more pronounced in the histoid form because the T and B cells showed greater infiltration than those in the lepromatous form. The activation of dendritic and Langerhans cells is similar in both forms. The spindle cells likely belong to the macrophage population, thus maintaining phagocytic ability. The quantities of pseudocapsules and bacilli are similar and cannot serve as criteria for diagnosis.</p>
  a0305-7518