02485nas a2200373   4500000000100000008004100001653001200042653002600054653001900080653002300099653002700122653002200149653002000171653001100191653001800202653000900220653002400229653001800253653003100271653001700302100001400319700001200333700001500345700001000360700001600370700001500386700001400401245012700415856005800542300001200600490000800612520147700620022001402097       2011                            d10aAnimals10aAntitubercular Agents10aBacterial Load10aBacterial Vaccines10aDisease Models, Animal10aDrug Combinations10aDrug Resistance10aHumans10aImmunotherapy10aMice10aMice, Inbred BALB C10aMycobacterium10aMycobacterium tuberculosis10aTuberculosis1 aFaujdar J1 aGupta P1 aNatrajan M1 aDas R1 aChauhan D S1 aKatoch V M1 aGupta U D00aMycobacterium indicus pranii as stand-alone or adjunct immunotherapeutic in treatment of experimental animal tuberculosis.  uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3249969/  a696-7030 v1343 a<p><strong>BACKGROUND &amp; OBJECTIVES: </strong>Mycobacterium w (M.w) is a saprophytic cultivable mycobacterium and shares several antigens with M. tuberculosis. It has shown good immunomodulation in leprosy patients. Hence in the present study, the efficacy of M.w immunotherapy, alone or in combination with multi drug chemotherapeutic regimens was investigated against drug sensitive M. tuberculosis H37Rv and three clinical isolates with variable degree of drug resistance in mice.</p>

<p><strong>METHODS: </strong>BALB/c mice were infected with M. tuberculosis H37Rv (susceptible to all first and second line drugs) and three clinical isolates taken from the epository of the Institute. The dose of 200 bacilli was used for infection via respiratory route in an aerosol chamber. Chemotherapy (5 days/wk) was given one month after infection and the vaccinated group was given a dose of 1x107 bacilli by subcutaneous route. Bacterial load was measured at 4 and 6 wk after initiation of chemotherapy.</p>

<p><strong>RESULTS: </strong>M.w when given along with chemotherapy (4 and 6 wk) led to a greater reduction in the bacterial load in lungs and other organs of TB infected animals compared to. However, the reduction was significantly (P&lt;0.05) more in terms of colony forming units (cfu) in both organs (lungs and spleen).</p>

<p><strong>CONCLUSION: </strong>M.w (as immunomodulator) has beneficial therapeutic effect as an adjunct to chemotherapy.</p>
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