02523nas a2200397   4500000000100000008004100001653001200042653001400054653001900068653001400087653001400101653001100115653001400126653001600140653000900156653001600165653000900181653002300190653001800213653001600231653001400247653002600261653002800287100001200315700001400327700001200341700001400353700001600367700001500383700001400398245014100412300001300553490000700566520153800573022001402111       2012                            d10aAnimals10aApoptosis10aBacterial Load10aCell Line10aCytokines10aFemale10aGranuloma10aGuinea Pigs10aLung10aMacrophages10aMice10aMice, Inbred C57BL10aMycobacterium10aNeutrophils10aTh1 Cells10aTuberculosis Vaccines10aTuberculosis, Pulmonary1 aGupta A1 aAhmad F J1 aAhmad F1 aGupta U D1 aNatarajan M1 aKatoch V M1 aBhaskar S00aProtective efficacy of Mycobacterium indicus pranii against tuberculosis and underlying local lung immune responses in guinea pig model.  a6198-2090 v303 a<p>Tuberculosis kills two million people each year. As the current vaccine BCG fails to prevent adult cases of TB, an improved vaccine and/or vaccination strategy is urgently needed to combat TB. Previously we reported the higher protective efficacy of Mycobacterium indicus pranii (MIP), formerly known as Mycobacterium w (M.w) as compared to BCG in murine model of TB. In this study we further evaluated the protective efficacy of MIP in guinea pig model of TB. Modulation of post infection immune response was analyzed in the lungs of MIP immunized and control groups. We found reduced bacterial loads, improved pathology and organized granulomatous response at different post infection time points in the MIP-immunized group as compared to the BCG-immunized group. Combined results suggest that MIP-immunization results in heightened protective Th1 response as compared to BCG group, early after infection with M.tb and a balanced Th1 versus immunosuppressive response at late chronic stage of infection. The study demonstrates the higher antigen presenting cells function both inside the granuloma as well as in the single cell suspension of the lung in the MIP-immunized group. We further demonstrate that live MIP is safe to use in vivo as we observed quick clearance of MIP from the body and no untoward reaction was found. Aerosol route of immunization provided higher protection. Further this study provides evidence that MIP-immunization gives significantly better long term protection as compared to BCG against TB.</p>
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