02210nas a2200445   4500000000100000008004100001260001600042653001500058653001000073653001800083653001100101653001600112653001700128653001100145653001100156653003000167653002500197653001800222653001200240653000900252653001600261653002500277653001300302653002000315653001800335100001600353700001400369700001400383700001100397700001400408700001400422700001400436700001300450245012800463300001000591490000800601050001800609520112300627022001401750       2002                            d  c2002 Jul 1510aAdolescent10aAdult10aAntigens, CD110aBiopsy10aCD3 Complex10aCD8 Antigens10aFemale10aHumans10aHypersensitivity, Delayed10aImmunohistochemistry10aKi-67 Antigen10aleprosy10aMale10aMiddle Aged10aMycobacterium leprae10aNeuritis10aTuberculin Test10aWound Healing1 aSiddiqui RM1 aMoreira A1 aNegesse Y1 aTaye G1 aHanekom W1 aHaslett P1 aBritton S1 aKaplan G00aLocal nerve damage in leprosy does not lead to an impaired cellular immune response or decreased wound healing in the skin.  a260-50 v186  aSIDDIQUI 20023 a<p>This study investigated whether peripheral nerve damage in patients with leprosy impairs local cellular immune responses, thereby reducing wound healing and leading to chronic skin ulceration. Anesthetic and contralateral sensitive skin sites in 42 patients with leprosy were compared for delayed-type hypersensitivity responses to purified protein derivative (PPD) of tuberculin. Leukocyte recruitment, epidermal activation, keratinocyte proliferation, and rates of wound healing after skin biopsy were compared. No significant differences in PPD-induced induration, epidermal activation and thickening or numbers of total T cells, CD8+ T cells, CD1a+ Langerhans cells, and proliferating Ki67+ keratinocytes were observed between anesthetic and sensitive skin sites. Similarly, rates of wound healing over 5 days after skin biopsy did not differ significantly. Thus, local leprosy-associated anesthesia does not appear to contribute to local immune compromise or impaired wound healing. Rather, chronic cutaneous ulceration in leprosy most likely results from repeated trauma associated with loss of sensation.</p>  a0022-1899