01433nas a2200253   4500000000100000008004100001260001300042653002600055653002700081653002400108653003800132653001100170653001200181653002500193653003200218100001400250245013400264856005100398300000900449490000700458050003200465520066800497022001401165       2002                            d  c2002 Mar10aAntibodies, Bacterial10aAntibodies, Monoclonal10aAntigens, Bacterial10aEnzyme-Linked Immunosorbent Assay10aHumans10aleprosy10aMycobacterium leprae10aSensitivity and Specificity1 aParkash O00aProgress towards development of immunoassays for detection of Mycobacterium leprae infection, employing 35kDa antigen: an update.  uhttps://leprosyreview.org/article/73/1/00-0003  a9-190 v73  aInfolep Library - available3 a<p>The 35 kDa antigen of Mycobacterium leprae is a membrane component that contains both B and T-cell stimulating epitopes. Monoclonal antibodies, primarily specific to M. leprae, have been developed against this antigen. Moreover, this antigen has been genetically engineered. Using recombinant 35 kDa antigen and/or a monoclonal antibody against an epitope on 35 kDa, a variety of antibody/antigen detecting tests have been described for detection of M. leprae infection. 35 kDa protein also stimulates peripheral blood mononuclear cells (PBMCs) from the majority of paucibacillary (PB) patients. Approaches using combined antibody and T cell are discussed.</p>  a0305-7518