01415nas a2200325   4500000000100000008004100001260001300042653001000055653003000065653002000095653003800115653001100153653001300164653001200177653001600189653002500205653001900230100001500249700001800264700001000282700001600292700001700308700001400325245007400339856004100413300001100454490000700465520060300472022001401075       2001                            d  c2001 Jun10aAdult10aCation Transport Proteins10aGenetic Linkage10aGenetic Predisposition to Disease10aHumans10aLepromin10aleprosy10aMiddle Aged10aMycobacterium leprae10aNuclear Family1 aHatagima A1 aOpromolla D V1 aUra S1 aFeitosa M F1 aBeiguelman B1 aKrieger H00aNo evidence of linkage between Mitsuda reaction and the NRAMP1 locus.  uhttp://ila.ilsl.br/pdfs/v69n2a02.pdf  a99-1030 v693 a<p>Thirty sib-pairs were ascertained through unrelated lepromatous probands. They consisted of 22 healthy individuals and 8 leprosy patients. The Mitsuda reactions of all sibs were evaluated both macroscopically and histologically, and high molecular weight genomic DNA was extracted from the white blood cells of all sib-pairs. Three DNA polymorphisms identified by polymerase chain reaction (274C/T, D543N, 1729 + 55del4) were used as chromosome markers at the NRAMP1 locus. Sib-pair comparisons did not disclose any sign of close linkage between the Mitsuda reaction and the genetic markers.</p>  a0148-916X