01600nas a2200349   4500000000100000008004100001260001300042653001200055653001800067653003100085653001100116653002300127653001200150653000900162653002800171653002500199653002700224653003000251653003000281653001300311100001400324700001400338700001600352700001300368245009100381856005900472300001000531490000700541050003200548520065600580022001401236       2001                            d  c2001 Dec10aAnimals10aBase Sequence10aDrug Resistance, Microbial10aHumans10aLeprostatic Agents10aleprosy10aMice10aMolecular Sequence Data10aMycobacterium leprae10aOligonucleotide Probes10apolymerase chain reaction10aPredictive Value of Tests10aRifampin1 aHonoré N1 aRoche P W1 aGrosset J H1 aCole S T00aA method for rapid detection of rifampicin-resistant isolates of Mycobacterium leprae.  uhttp://leprev.ilsl.br/pdfs/2001/v72n4/pdf/v72n4a07.pdf  a441-80 v72  aInfolep Library - available3 a<p>A genotypic method for predicting rifampicin resistance in Mycobacterium leprae has been developed and rigorously tested on mouse footpad-derived and clinical specimens. A series of immobilized oligonucleotide capture probes can discriminate between wild type and mutant rpoB alleles, and positive controls are available for the most frequent mutation affecting Ser425. Two different non-radioactive detection formats have been tested with comparable success in both an industrialized and a developing country. The standardized procedure could now be used in a prospective study of potential rifampicin resistance among multibacillary patients.</p>  a0305-7518