01561nas a2200289   4500000000100000008004100001260001300042653001200055653001800067653002300085653002200108653001100130653001200141653002800153653002500181100001600206700001200222700001400234700001300248245005500261856005900316300001000375490000700385050003200392520083300424022001401257       2001                            d  c2001 Dec10aAnimals10aBase Sequence10aChromosome Mapping10aGenome, Bacterial10aHumans10aleprosy10aMolecular Sequence Data10aMycobacterium leprae1 aEiglmeier K1 aSimon S1 aGarnier T1 aCole S T00aThe integrated genome map of Mycobacterium leprae.  uhttp://leprev.ilsl.br/pdfs/2001/v72n4/pdf/v72n4a09.pdf  a462-90 v72  aInfolep Library - available3 a<p>The integrated map of the Mycobacterium leprae genome unveiled for the first time the genomic organization of this obligate intracellular parasite. Selected cosmid clones, isolated from a genomic library created in the cosmid vector Lorist6, were identified as representing nearly the complete genome and were subsequently used in the M. leprae genome sequencing project. Now a new version of the integrated map of M. leprae can be presented, combining the mapping results from the Lorist6 cosmids with data obtained from a second genomic library constructed in an Escherichia coli-mycobacterium shuttle cosmid, pYUB18. More than 98% of the M. leprae genome is now covered by overlapping large insert genomic clones representing a renewable source of well defined DNA segments and a powerful tool for functional genomics.</p>  a0305-7518