01632nas a2200337   4500000000100000008004100001260001300042653001100055653001700066653002000083653002200103653001500125653002900140653001800169653001200187653003000199653001100229653003700240653001000277653002400287100001700311700001500328700001800343700001500361245012300376856007900499300000900578490000700587520068600594022001401280       1975                            d  c1975 Feb10aUganda10aTuberculosis10aTuberculin Test10aTropical Medicine10aSkin Tests10aMycobacterium Infections10aMycobacterium10aleprosy10aHypersensitivity, Delayed10aHumans10aDemocratic Republic of the Congo10aChild10aAntigens, Bacterial1 aStanford J L1 aRevill W D1 aGunthorpe W J1 aGrange J M00aThe production and preliminary investigation of Burulin, a new skin test reagent for Mycobacterium ulcerans infection.  uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2131542/pdf/jhyg00070-0015.pdf  a7-160 v743 a<p>The preparation of a skin test antigen from Mycobacterium ulcerans by ultrasonic disintegration and filtration is described. The reagent, called Burulin, was tested in Africa in normal school children, and in patients with leprosy, tuberculosis or M. ulcerans disease. Those with tuberculosis or M. ulcerans disease were simultaneously tested with Tuberculin PPD. Burulin was found to be highly specific for patients in the reactive stage of M. ulcerans disease, and there was no cross-reaction in patients with other mycobacterioses. On the other hand, the majority of patients with M. ulcerans disease reacting to Burulin also produce positive reactions to Tuberculin PPD.</p>  a0022-1724