00893nas a2200181   4500000000100000008004100001653001200042653002500054653002700079653002500106653001700131100001600148700001300164700001300177245012400190520038300314022001400697       2014                            d10aleprosy10aMycobacterium leprae10aGentleMACS dissociator10amouse footpad tissue10apurification1 aWilliams DL1 aAdams LW1 aLahiri R00aSemi-automated Protocol for Purification of Mycobacterium leprae From Tissues Using the gentleMACS™ Octo Dissociator.3 a<p>Mycobacterium leprae, etiologic agent of leprosy, is propagated in athymic nude mouse footpads (FP). The current purification protocol is tedious and physically demanding. A simpler, semi-automated protocol was developed using gentleMACS™ Octo Dissociator. The gentleMACS protocol provided a very effective means for purification of highly viable M. leprae from tissue.</p>  a1872-8359