02160nas a2200445   4500000000100000008004100001260001300042653001000055653001200065653002500077653001100102653001900113653003800132653002000170653001100190653001900201653002500220653000900245653001100254653001600265653003000281653002600311653004600337653002200383653003000405653003200435653001600467100002200483700002300505700002100528700002000549700002400569700002100593245011200614856005100726300000900777490000700786520090700793022001401700       2012                            d  c2012 Mar10aAdult10aAlleles10aCase-Control Studies10aFemale10aGene Frequency10aGenetic Predisposition to Disease10aGenetic Testing10aHumans10aInterleukin-1010aLeprosy, lepromatous10aMale10aMexico10aMiddle Aged10apolymerase chain reaction10aPolymorphism, Genetic10aPolymorphism, Restriction Fragment Length10aPopulation Groups10aPromoter Regions, Genetic10aTumor Necrosis Factor-alpha10aYoung Adult1 aVelarde Félix JS1 aCázarez-Salazar S1 aRíos-Tostado JJ1 aFlores-Garcia A1 aRangel-Villalobos H1 aMurillo-Llanes J00aLack of effects of the TNF-alpha and IL-10 gene polymorphisms in Mexican patients with lepromatous leprosy.  uhttps://leprosyreview.org/article/83/1/03-4039  a34-90 v833 a<p>Several human genetic variants have been associated with susceptibility or resistance to leprosy. The aim of this study was to assess whether gene polymorphisms of -308 G/A TNF-alpha and -819 T/C IL-10 are associated with lepromatous leprosy in Mexican mestizos patients from northwest Mexico. We genotyped these polymorphisms by means of polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLPs) in 68 patients with lepromatous leprosy and 144 healthy Mexican Mestizos controls. We found that the -308G TNF-alpha allele was predominant in both cases (94.3%) and controls (92.3%) without statistical significance and the frequencies of -819C IL-10 allele were also similar for the cases (56.0%) and controls (59.0%). These negative findings suggest that other genes or polymorphisms may be important in the susceptibility to leprosy infection in the Mexican mestizos.</p>  a0305-7518