01658nas a2200277   4500000000100000008004100001260000900042653001200051653001200063653002100075653001800096653001900114653001100133653002300144653001800167653000900185100001300194700001300207700001500220245006000235300001100295490001200306050001600318520103200334022001401366       1992                            d  c199210aAnimals10aCadmium10aCarrier Proteins10aCell Division10aConcanavalin A10aHumans10aNutritional Status10aT-Lymphocytes10aZinc1 aOhsawa M1 aOtsuka F1 aSugizaki S00aZinc status in proliferative response of T lymphocytes.  a518-210 vSpec No  aOHSAWA 19923 a<p>The in vitro proliferation of T lymphocytes are highly susceptible to Zn deprivation and Cd addition. In order to define Zn status in T cell proliferation, toxicological and biochemical situation of Zn in proliferative response of T cells was investigated by use of mouse spleen cell cultures stimulated by T cell-mitogen, Con A. The inhibitory effect of Cd on T cell proliferation was protected specifically by Zn. The protection by Zn was effective when Zn was added within 16 h after Con A stimulation. Zn addition affected neither Cd content in cells nor induction of Cd-thionein. These findings indicate that Zn-dependent processes expressed before the S phase of cell cycle are critical for T cell proliferation and are targets susceptible to Cd. Moreover, electrophoretical analysis showed two unknown Zn-binding proteins (49 and 100 KD) induced in spleen cells incubated with Con A and radioactive Zn. These Zn-binding proteins may be associated with the Zn-dependent processes critical for T cell proliferation.</p>  a0301-4800