02033nas a2200265   4500000000100000008004100001653000900042653000900051653001200060653000900072653001100081100001000092700001400102700001300116700001400129700001400143700001900157700001500176700001900191245010100210300000600311050002100317520141500338022001401753       2013                            d10aTLR210aMLSA10aleprosy10aIL1010aIFN γ1 aJain 1 aSubbanna 1 aVenkata 1 aKamakshi 1 aSiva Sai 1 aVijayaLakshmi 1 aAnandaraj 1 aSuryadevara NC00aInfluence of Intron II Microsatellite Polymorphism in Human Toll-like Receptor 2 Gene in Leprosy  a-  aSURYADEVARA 20133 aLeprosy is a chronic granulomatous infection caused by the obligate intracellular organism Mycobacterium leprae. \{TLR2\} plays a key role when activated by M. leprae lipoproteins initiating protective responses which induce bacterial killing and therefore control of disease spread. Microsatellite polymorphisms in intron2 of \{TLR2\} gene have been reported to be associated with development of clinical features of several infectious diseases. The study aims to evaluate the influence of \{GT\} microsatellite on the expression of \{TLR2\} which could make humans prone to M.Leprae infections. A total of 279 individuals were enrolled in the study, 88 were leprosy patients, 95 were house hold contacts (HHC) and 96 were healthy controls (HC). Genotyping was done using PCR-Sequencing method. \{TLR2\} mRNA expression was analyzed by RT-PCR. IL-10 and IFN-γ levels were measured using \{ELISA\} in \{MLSA\} stimulated cell culture supernatants. Statistical analysis was performed using Chi-Square (χ2) test and t-tests. Allele/genotype of \{TLR2\} microsatellite which includes longer \{GT\} repeats was associated with low \{TLR2\} mRNA expression and high IL-10 production while that including shorter \{GT\} repeats was associated with high \{TLR2\} mRNA expression and low IL-10 production. High \{IL10\} producing allele of \{TLR2\} microsatellite might predispose house hold contacts to leprosy. "
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