02614nas a2200505   4500000000100000008004100001260001600042653001000058653000900068653001700077653002500094653002000119653001800139653003600157653001100193653001100204653002100215653001900236653002600255653001200281653002800293653002600321653001600347653000900363653001600372653002500388653004300413653004400456653001900500653002500519653002900544653003200573100002000605700001100625700001400636700001300650700002100663700001800684700002100702245013200723300001000855490000700865520122200872022001402094       2001                            d  c2001 Feb 0110aAdult10aAged10aCD56 Antigen10aCell Differentiation10aCells, Cultured10aChaperonin 1010aCytotoxicity Tests, Immunologic10aFemale10aHumans10aInterferon-gamma10aInterleukin-1210aKiller Cells, Natural10aleprosy10aLeukocytes, Mononuclear10aLymphocyte Activation10aMacrophages10aMale10aMiddle Aged10aMycobacterium leprae10aReceptors, Antigen, T-Cell, alpha-beta10aReceptors, Antigen, T-Cell, gamma-delta10aReceptors, IgG10aT-Lymphocyte Subsets10aT-Lymphocytes, Cytotoxic10aTumor Necrosis Factor-alpha1 aDe La Barrera S1 aFink S1 aFiniasz M1 aAleman M1 aHelena Fariña M1 aPizzariello G1 aCarmen Sasiain M00aLysis of autologous macrophages pulsed with hsp10 from Mycobacterium leprae is associated to the absence of bacilli in leprosy.  a55-620 v763 a<p>Peripheral blood mononuclear cells from leprosy patients and normal individuals were analysed for their ability to lyse autologous macrophages pulsed with the Mycobacterium leprae 10 kDa heat shock protein (hsp10), an antigen considered to have an important role in the protective responses in leprosy. Strong cytotoxic responses, with an involvement of gammadelta T and class-I and class-II restricted alphabeta T cells and/or CD16+56+ cells, were observed in normal individuals, paucibacillary (PB) and those multibacillary (MB) patients with undetectable bacillary load. On the contrary, only a weak class-II restricted cytotoxic response was observed in those MB patients with positive bacillary load (MB(+)). Simultaneous addition of IFNgamma plus TNFalpha and IL-12 during hsp10 stimulation could partially upregulate the low cytotoxic response observed in MB(+) by enhancing class-II restricted T cell activity and by development of gammadelta T and/or CD16+56+ cell activity. Our results suggest that the ability to mount an effective cytotoxic response against hsp10-pulsed macrophages in leprosy patients is closely related to the patient's bacterial load and not to the clinical form of the disease.</p>  a0165-2478