01563nas a2200361   4500000000100000008004100001260001600042653001200058653002400070653001600094653001800110653001600128653001200144653000900156653000900165653002400174653002300198653002400221653002500245653001300270653001100283653002400294100001200318700001500330700001600345700001200361700001300373245019500386300001200581490000700593520058700600022001401187       2001                            d  c2001 Feb 2810aAnimals10aAntigens, Bacterial10aBCG Vaccine10aBase Sequence10aDNA Primers10aleprosy10aMale10aMice10aMice, Inbred BALB C10aMice, Inbred C57BL10aMycobacterium bovis10aMycobacterium leprae10aPlasmids10aSpleen10aVaccines, Synthetic1 aOhara N1 aMatsuoka M1 aNomaguchi H1 aNaito M1 aYamada T00aProtective responses against experimental Mycobacterium leprae infection in mice induced by recombinant Bacillus Calmette-Guérin over-producing three putative protective antigen candidates.  a1906-100 v193 a<p>The components of Ag85 (Ag85A, Ag85B, and Ag85C) are putative protective antigen candidates against mycobacterial infection. A recombinant Mycobacterium bovis Bacillus Calmette-Guérin (rBCG) over-producing Ag85A, Ag85B, and MPB51 (rBCG/BA51) was constructed. rBCG/BA51 could secrete these antigens at levels more than five times higher than parental BCG. Immunization of C57BL/6 and BALB/c mice with this rBCG reduced the multiplication of Mycobacterium leprae in the foot pads of both strains of mice. The inhibition by rBCG/BA51 was more evident than that by parental BCG.</p>  a0264-410X