02779nas a2200589   4500000000100000008004100001260006000042653001500102653001000117653000900127653002200136653002600158653001100184653001100195653001900206653003100225653001100256653002100267653001100288653001200299653000900311653001600320653002300336653002500359653001900384653003600403653001500439653002400454653001500478653002700493653000900520653001600529100001800545700001300563700001300576700001500589700001400604700001200618700001300630700001400643700001600657700001500673700001200688700001400700700001800714245011200732856007300844300001100917490000700928520124000935022001402175       2012                            d  c2012 JunbAmerican Society for MicrobiologyaWashington10aAdolescent10aAdult10aAged10aAged, 80 and over10aAnti-Bacterial Agents10aBiopsy10aBrazil10aDNA, Bacterial10aDrug Resistance, Bacterial10aFemale10aGenes, Bacterial10aHumans10aleprosy10aMale10aMiddle Aged10aMutation, Missense10aMycobacterium leprae10aPoint Mutation10aPolymorphism, Single Nucleotide10aPrevalence10aProspective Studies10aRecurrence10aSequence Analysis, DNA10aSkin10aYoung Adult1 aSilva Rocha A1 aCunha MG1 aDiniz LM1 aSalgado CG1 aAires MAP1 aNery JA1 aGallo EN1 aMiranda A1 aMagnanini M1 aMatsuoka M1 aSarno E1 aSuffys PN1 aOliveira MLWD00aDrug and multidrug resistance among Mycobacterium leprae isolates from Brazilian relapsed leprosy patients.  uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3372169/pdf/zjm1912.pdf  a1912-70 v503 a<p>Skin biopsy samples from 145 relapse leprosy cases and from five different regions in Brazil were submitted for sequence analysis of part of the genes associated with Mycobacterium leprae drug resistance. Single nucleotide polymorphisms (SNPs) in these genes were observed in M. leprae from 4 out of 92 cases with positive amplification (4.3%) and included a case with a mutation in rpoB only, another sample with SNPs in both folP1 and rpoB, and two cases showing mutations in folP1, rpoB, and gyrA, suggesting the existence of multidrug resistance (MDR). The nature of the mutations was as reported in earlier studies, being CCC to CGC in codon 55 in folP (Pro to Arg), while in the case of rpoB, all mutations occurred at codon 531, with two being a transition of TCG to ATG (Ser to Met), one TCG to TTC (Ser to Phe), and one TCG to TTG (Ser to Leu). The two cases with mutations in gyrA changed from GCA to GTA (Ala to Val) in codon 91. The median time from cure to relapse diagnosis was 9.45 years but was significantly shorter in patients with mutations (3.26 years; P = 0.0038). More than 70% of the relapses were multibacillary, including three of the mutation-carrying cases; one MDR relapse patient was paucibacillary.</p>  a1098-660X