02218nas a2200289   4500000000100000008004100001260002000042653002100062653001100083653001700094653001500111653001200126653002100138653003200159100001200191700001300203700001200216700001300228700001100241245008900252856004600341300001300387490000700400050001600407520149100423022001401914       2010                            d  c02/2010bScielo10aSkin smears; PCR10aSaliva10aPeriodontium10atratamento10aleprosy10aFull text online10aBacteriological examination1 aNaveca 1 aAbdalla 1 aSantos 1 aCollado 1 aCunha 00aMycobacterium leprae in the periodontium, saliva and skin smears of leprosy patients  uhttp://www.scielo.br/pdf/roc/v25n2/08.pdf  a148–530 v25  aABDALLA20103 aPurpose: To verify the presence of M. leprae in the periodontium, saliva and skin slit smears of leprosy patients. To correlate bacteriological and molecular findings with clinical data and compare laboratory techniques.
Methods: A cross-sectional study was designed to use bacteriological (baciloscopy) and molecular (PCR) parameters to detect M. leprae in exudates of the gingival sulcus/periodontium pocket, saliva and skin slit smears from multiple clinical forms of leprosy patients without previous treatment.
Results: The study included 48 leprosy patients with 15 multibacillary (MB) cases and 33 paucibacillary (PB) cases. The diagnosis of MB was confirmed through bacteriological examination and PCR results from skin slit smears. A total of 16 (48.5%) PB patients were PCR positive only. Four PB patients with negative PCR skin smears were PCR positive for the periodontium and saliva, with 2 cases and 1 case, respectively. No periodontium or saliva samples had positive bacteriological results.
Conclusion: There was no correlation between periodontal disease and the presence of M. leprae. Bacteriological examination did not prove to be an efficient technique for the analysis of saliva and periodontium samples. PCR analysis of skin smears was more efficient at diagnosing PB patients than bacteriological examination. PCR positive results for the detection of M. leprae in PB patients can be increased by collecting slit skin smears, periodontium and saliva samples.  a1980-6523