03311nas a2200433   4500000000100000008004100001260004100042653002600083653002900109653001700138653001300155653002300168653002300191653002000214653003900234653003700273653004100310653001100351653002100362653002800383653001300411653003200424653001300456653001900469653002200488653001300510653001600523100001400539700001400553700001800567700001200585700001700597700001100614245008800625300001100713490000700724520213200731022001402863       2012                            d  c2012 AprbInforma HealthcareaLondon10aAnti-Bacterial Agents10aAnti-Inflammatory Agents10aAntioxidants10aBacteria10aBiphenyl Compounds10aCell Proliferation10aCells, Cultured10aDisk Diffusion Antimicrobial Tests10aDose-Response Relationship, Drug10aGas Chromatography-Mass Spectrometry10aHumans10aHydrocharitaceae10aLeukocytes, Mononuclear10aMethanol10aMicrobial Sensitivity Tests10aPicrates10aPlant Extracts10aPlants, Medicinal10aSolvents10aSuperoxides1 aYuvaraj N1 aKanmani P1 aSatishkumar R1 aPaari A1 aPattukumar V1 aArul V00aSeagrass as a potential source of natural antioxidant and anti-inflammatory agents.  a458-670 v503 a<p><b>CONTEXT: </b>Halophila spp. is a strong medicine against malaria and skin diseases and is found to be very effective in early stages of leprosy. Seagrasses are nutraceutical in nature and therefore of importance as food supplements.</p><p><b>OBJECTIVE: </b>The antibacterial, antioxidant, and anti-inflammatory activities of Halophila ovalis R. Br. Hooke (Hydrocharitaceae) methanol extract were investigated and the chemical constituents of purified fractions were analyzed.</p><p><b>MATERIALS AND METHODS: </b>Plant materials were collected from Pondicherry coastal line, and antimicrobial screening of crude extract, and purified fractions was carried out by the disc diffusion method and the minimum inhibitory concentration (MICs) of the purified fractions and reference antibiotics were determined by microdilution method. Antioxidant and anti-inflammatory activities were investigated in vitro. Chemical constituents of purified fractions V and VI were analyzed by gas chromatography-mass spectrometry (GC-MS), and the phytochemicals were quantitatively determined.</p><p><b>RESULTS: </b>Methanol extract inhibited the growth of Bacillus cereus at a minimum inhibitory concentration of 50 µg/mL and other Gram-negative pathogens at 75 µg/ml, except Vibrio vulnificus. Reducing power and total antioxidant level increased with increasing extract concentration. H. ovalis exhibited strong scavenging activity on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide radicals at IC(50) of 0.13 and 0.65 mg/mL, respectively. Methanol extract of H. ovalis showed noticeable anti-inflammatory activity at IC(50) of 78.72 µg/mL. The GC-MS analysis of H. ovalis revealed the presence of triacylglycerols as major components in purified fractions. Quantitative analysis of phytochemicals revealed that phenols are rich in seagrass H. ovalis.</p><p><b>DISCUSSION AND CONCLUSION: </b>These findings demonstrated that the methanol extract of H. ovalis exhibited appreciable antibacterial, noticeable antioxidant, and anti-inflammatory activities, and thus could be use as a potential source for natural health products.</p>  a1744-5116