02379nas a2200505   4500000000100000008004100001260005300042653001000095653000900105653002300114653001100137653001100148653001100159653001300170653001100183653001200194653000900206653001600215653002100231653002500252653001500277653003900292653002700331653000900358100001800367700002400385700001600409700001200425700001500437700001400452700001900466700001400485700001500499700001500514700002100529700001200550700001800562700001400580245016700594856007000761300001100831490000700842520101000849022001401859       2011                            d  c2011 OctbSociety for General Microbiologyas.l.10aAdult10aAged10aBacterial Proteins10aBiopsy10aBrazil10aFemale10agenotype10aHumans10aleprosy10aMale10aMiddle Aged10aMolecular Typing10aMycobacterium leprae10aRecurrence10aRepetitive Sequences, Nucleic Acid10aSequence Analysis, DNA10aSkin1 aSilva Rocha A1 aCunha Dos Santos AA1 aPignataro P1 aNery JA1 aMiranda AB1 aSoares DF1 aBrum Fontes AN1 aMiranda A1 aFerreira H1 aBoéchat N1 aNovisck Gallo ME1 aSarno E1 aOliveira MLWD1 aSuffys PN00aGenotyping of Mycobacterium leprae from Brazilian leprosy patients suggests the occurrence of reinfection or of bacterial population shift during disease relapse.  uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347867/pdf/1441.pdf  a1441-60 v603 a<p>We performed genotyping of Mycobacterium leprae present in skin biopsy samples that were collected during the first and the second disease occurrences from eight leprosy patients, seven of whom were diagnosed as suffering from disease relapse. Sequence analysis of part of the M. leprae rpoB, folP1, gyrB and gyrA genes did not show genetic change that supported the presence of drug-resistant bacilli. However, we observed a synonymous nucleotide change at position 297 of gyrA among five of these patients, one presenting C to T (CgyrAT) and four presenting T to C (TgyrAC) at this position. Additional genotyping by analysis of the four short tandem repeats GAA, GTA9, AT17 and TA18 showed that the gyrA single nucleotide polymorphism change was accompanied by a change in short tandem repeat genotype. Our data suggest that leprosy relapse in these patients, living in an area endemic for leprosy, could be caused by M. leprae with a genotype different from the one that caused initial disease.</p>  a1473-5644