02262nas a2200433 4500000000100000008004100001260001300042653002400055653002100079653001500100653001200115653001400127653003800141653001600179653001100195653002200206653002100228653001900249653001200268653002900280653002100309653003200330100001600362700001200378700002300390700001700413700001800430700001800448700001500466700001300481700001600494700001200510245009300522856007600615300001100691490000700702520110500709022001401814 2011 d c2011 Oct 10 aAntigens, Bacterial 10 aBiological Assay 10 aBiomarkers 10 aBuffers 10 aCytokines 10 aEnzyme-Linked Immunosorbent Assay 10 aGlycolipids 10 aHumans 10 aImmunity, Humoral 10 aInterferon-gamma 10 aInterleukin-10 10 aleprosy 10 aMycobacterium Infections 10 aReference Values 10 aSensitivity and Specificity 1 aCorstjens P 1 aDood CJ 1 aPloeg-van Schip JJ 1 aWiesmeijer K 1 aRiuttamäki T 1 aMeijgaarden K 1 aSpencer JS 1 aTanke HJ 1 aOttenhoff T 1 aGeluk A 00 aLateral flow assay for simultaneous detection of cellular- and humoral immune responses. uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3177995/pdf/nihms310566.pdf a1241-6 0 v44 3 aOBJECTIVE: The development of a cytokine detection assay suitable for detection of multiple biomarkers for improved diagnosis of mycobacterial diseases.
DESIGN AND METHODS: A lateral flow (LF) assay to detect IL-10 was developed utilizing the up-converting phosphor (UCP) reporter-technology. The assay was evaluated using blood samples of leprosy patients. Multiplex applications were explored targeting: 1) IL-10 and IFN-γ in assay buffer; 2) IL-10 and anti-phenolic glycolipid (PGL-I) antibodies in serum from leprosy patients.
RESULTS: Detection of IL-10 below the targeted level of 100pg/mL in serum was shown. Comparison with ELISA showed a quantitative correlation with R(2) value of 0.92. Multiplexing of cytokines and simultaneous detection of cytokine and antibody was demonstrated.
CONCLUSIONS: The UCP-LF IL-10 assay is a user-friendly, rapid alternative for IL-10 ELISAs, suitable for multiplex detection of different cytokines and can be merged with antibody-detection assays to simultaneously detect cellular- and humoral immunity.
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