02467nas a2200433   4500000000100000008004100001260001300042653001500055653001100070653001100081653001400092653001100106653001100117653002500128653002100153653001900174653002400193653002500217653002500242653000900267653001600276653003400292653003000326653003600356653003200392100001600424700001400440700001500454700001400469700001000483700001500493700002000508245013200528856005100660300001000711490000700721520129100728022001402019       2011                            d  c2011 Mar10aBiomarkers10aBiopsy10aBrazil10aCytokines10aFemale10aHumans10aImmunohistochemistry10aInterferon-gamma10aInterleukin-1010aLeprosy, Borderline10aLeprosy, lepromatous10aLeprosy, Tuberculoid10aMale10aMiddle Aged10aNitric Oxide Synthase Type II10aStatistics, Nonparametric10aTransforming Growth Factor beta10aTumor Necrosis Factor-alpha1 aVenturini J1 aSoares CT1 aBelone AFF1 aBarreto J1 aUra S1 aLauris JRP1 aVilani-Moreno F00aIn vitro and skin lesion cytokine profile in Brazilian patients with borderline tuberculoid and borderline lepromatous leprosy.  uhttps://leprosyreview.org/article/82/1/02-5035  a25-350 v823 a<p><b>OBJECTIVE: </b>We investigated the in vitro and skin lesions production of cytokines in non-treated borderline tuberculoid (BT) and borderline lepromatous (BL) patients.</p><p><b>PATIENTS AND METHODS: </b>Seven untreated, non-reactional BT patients and 12 untreated, non-reactional BL patients were studied. Levels of the cytokines IFN-gamma, IL-10, TGF-beta1 and TNF-alpha were measured in supernantant of peripheral blood mononuclear cells (PBMC) cultures, stimulated with specific M. leprae antigen (sonicated and whole). The cytokines iNOS, IL-10 and TGF-beta1 were detected by immunohistochemistry in skin biopsies.</p><p><b>RESULTS: </b>BT patients produced higher levels of IFN-gamma than BL patients; iNOS expression in skin lesions was also higher in BT patients. TGF-beta1 was detected in more cells in BL patients; IL-10 expression was similar in both groups. There was a negative correlation between iNOS and TGF-beta1 expression in skin biopsies, positive correlation between TGF-beta1 in skin lesions and bacillary index, as well as positive correlation between iNOS detected in skin biopsies and PBMC IFN-gamma production.</p><p><b>CONCLUSIONS: </b>The BT patients had a mainly a Th1-profile of cytokines in their skin lesions and BL patients had a Th2 profile.</p>  a0305-7518