02488nas a2200361   4500000000100000008004100001260001300042653001200055653001700067653001400084653002500098653002600123653002900149653001100178653001600189653001400205653001600219653000900235653002100244653002300265653001400288653002900302653003100331653001700362100001500379700001500394700001200409245019600421300001200617490000700629520147600636022001402112       1975                            d  c1975 Nov10aAnimals10aCell Nucleus10aCytoplasm10aCytoplasmic Granules10aEndoplasmic Reticulum10aExudates and Transudates10aFemale10aLymphocytes10aLysosomes10aMacrophages10aMice10aMice, Inbred C3H10aMice, Inbred C57BL10aMonocytes10aMycobacterium Infections10aMycobacterium lepraemurium10aTime Factors1 aHaugen O A1 aSkjorten F1 aCloss O00aExperimental murine leprosy. 8. Ultrastructural features of the inflammatory exudate and bacterial morphology in C3H and C57BL mice after foot-pad inoculation with Mycobacterium lepraemurium.  a693-7030 v833 a<p>Mice of the inbred strains C57BL and C3H were inoculated in the foot-pads with Mycobacterium lepraemurium (MLM) and the inflammatory reaction was studied using light and electron microscopy. In C57BL mice a granulomatous reaction developed 3-4 weeks after inoculation. The inflammatory exudate at this stage showed numerous lymphocytes, monocytes and macrophages. The latter cell type often contained many lysosomes and appeared activated. The bacilli which were all within phagosomes showed extensive electron dense aggregates of the cytoplasm suggesting severe damage. Lymphocytes and macrophages in close contact with each other were often observed. In macrophages which contained damaged bacilli, spherical lipid-like bodies surrounded by granular endoplasmic reticulum were observed. It is suggested that this cell product could be of some significance for the bactericidal function of the macrophage. Contrary to these findings, the cellular infiltrate developing in C3H mice showed no lymphocytes and consisted exclusively of macrophages. These were all heavily loaded with bacilli. The vast majority of bacilli encountered in this strain was morphologically intact and presumably viable. Lipid-like bodies similar to those observed in infected C57BL macrophages were not encountered in C3H mice. It is concluded that unless the infected macrophages become immunologically activited they are unable to cause bacterial damage or to inhibit the growth of MLM.</p>  a0365-4184