01914nas a2200397   4500000000100000008004100001260001300042653002300055653003200078653002100110653002200131653002200153653001300175653001400188653001100202653001200213653002500225653003000250653002700280653001700307100001500324700001200339700001000351700001300361700001300374700001500387700001600402700001200418700001800430245009100448856004100539300001000580490000700590520090500597022001401502       2000                            d  c2000 Jun10aBacterial Proteins10aBacterial Typing Techniques10aGenes, Bacterial10aGenetic Variation10aGenome, Bacterial10agenotype10aGeography10aHumans10aleprosy10aMycobacterium leprae10apolymerase chain reaction10aSequence Analysis, DNA10aSigma Factor1 aMatsuoka M1 aMaeda S1 aKai M1 aNakata N1 aChae G T1 aGillis T P1 aKobayashi K1 aIzumi S1 aKashiwabara Y00aMycobacterium leprae typing by genomic diversity and global distribution of genotypes.  uhttp://ila.ilsl.br/pdfs/v68n2a01.pdf  a121-80 v683 a<p>The genetic diversity and related global distribution of 51 Mycobacterium leprae isolates were studied. Isolates were obtained from leprosy patients from 12 geographically distinct regions of the world and two were obtained from nonhuman sources. Polymerase chain reaction (PCR) followed by DNA sequencing was performed targeting the rpoT gene of M. leprae. Isolates were classified into two groups based on the number of tandem repeats composed of 6 base pairs in the rpoT gene. Isolates from Japan (except Okinawa) and Korea belonged to one group, while those from Southeast Asian countries, Brazil, Haiti and Okinawa in Japan belonged to a second genotype. M. leprae obtained from two nonhuman sources (an armadillo and a mangabey monkey) revealed the latter genotype. These results demonstrate the genetic diversity of M. leprae and the related genotype-specific distribution in the world.</p>  a0148-916X