03092nas a2200421   4500000000100000008004100001260001300042653001500055653001000070653002200080653003100102653003100133653002000164653001000184653002300194653001100217653001900228653001100247653002100258653002100279653001900300653001800319653000900337653001900346100001100365700001200376700001500388700001300403700001400416700001100430700001300441700001200454245018200466300001200648490000800660520198800668022001402656       1998                            d  c1998 Dec10aAdolescent10aAdult10aBlood Circulation10aCD4-Positive T-Lymphocytes10aCD8-Positive T-Lymphocytes10aCells, Cultured10aChild10aDermatitis, Atopic10aFemale10aFlow Cytometry10aHumans10aImmunoglobulin E10aInterferon-gamma10aInterleukin-1310aInterleukin-410aMale10aRNA, Messenger1 aSato A1 aTsuji K1 aYamamura M1 aMorita Y1 aKanzaki H1 aTada J1 aMakino H1 aArata J00aIncreased type 2 cytokine expression by both CD4+ CD45RO+ T cells and CD8+ CD45RO+ T cells in blood circulation is associated with high serum IgE but not with atopic dermatitis.  a1079-840 v1113 a<p>Type 2 cytokines, such as interleukin-4 (IL-4) and IL-13, are associated with immunoglobulin E (IgE) production. This association has also been observed in CD8+ T cells from patients infected with leprosy and human immunodeficiency virus (HIV). Using intracellular cytokine staining and flow cytometry, the cytokine profile [IL-2, IL-4, IL-10, IL-13, and interferon (IFN)-gamma] of both CD4+ and CD8+ memory/effector T cells circulating in atopic dermatitis (AD) patients was investigated at the single cell level. The levels of type 2 cytokines in CD4+ T cells or CD8+ T cells in AD patients with high levels of serum IgE (AD-H), low levels of serum IgE (AD-L), and healthy controls were compared. Increased production of IL-4 and IL-13 in both CD4+ CD45RO+ T cells and CD8+ CD45RO+ T cells after 4 h in vitro stimulation with phorbol 12-myristate 13-acetate and ionomycin, was more prominent in AD-H patients than in AD-L patients or healthy controls, whereas IFN-gamma-producing CD4+ CD45RO+ T cells and CD8+ CD45RO+ T cells were relatively diminished in AD-H patients. CD4+ T cells and CD8 + T cells from AD-H patients, cultured for 48 h with phorbol 12-myristate 13-acetate and ionomycin, released larger amounts of IL-4 and IL-13 but smaller amounts of IFN-gamma than both types of cells from AD-L patients or healthy controls. In addition, when stimulated with immobilized anti-CD3 monoclonal antibody (MoAb) and anti-CD28 MoAb, CD4+ CD45RO+ T cells and CD8+ CD45RO+ T cells from AD-H patients contained more IL-4-producing cells but fewer IFN-gamma-producing cells compared with healthy controls. Finally, spontaneous mRNA expression of IL-4 in blood CD8+ CD45RO+ T cells isolated from AD-H patients was increased, as determined by reverse transcriptase-polymerase chain reaction. Therefore, in AD patients with high IgE levels, type 2 cytokine (IL-4 and IL-13) expression is associated with IgE production, in both CD4+ CD45RO+ T cell and CD8+ CD45RO+ T cell subsets.</p>  a0022-202X