01801nas a2200265   4500000000100000008004100001260001700042653001200059653003100071653001100102653001100113653001400124653001200138653000900150653001600159653002500175653001300200653001700213100001600230700001400246245010700260300001000367520114400377022001401521       1978                            d  c1978 Oct-Dec10aDapsone10aDrug Resistance, Microbial10aFemale10aHumans10aIsoniazid10aleprosy10aMale10aMiddle Aged10aMycobacterium leprae10aRifampin10aTime Factors1 aMurohashi T1 aYoshida K00aDrug sensitivity of M. leprae isolated from leprosy patients administered DDS for long period of time.  a13-283 a<p>Drug sensitivity was tested using liquid medium on three stains isolated from the subcutaneous nodules of L-type patients who have long been administered DDS alone. The results revealed that the first strain was resistant to DDS up to the concentration of 1.0 microgram/ml suggesting as if it were DDS dependent or enhanced strain, whereas the second strain was completely sensitive to DDS even at the lowest concentration of 0.01 microgram/ml suggesting possible inactivation of this drug in the host patient. The third strain was completely resistant to 0.1 microgram/ml, but sensitive to 1.0 microgram/ml of DDS, suggesting that the therapeutic effect can not be expected any more, when the strain becomes resistant to 0.1 microgram/ml of DDS. All of the three strains were sensitive to REP at the concentration of 0.01 microgram/ml, and the host patients of the former two strains showed rapid improvement of the clinical symptomes after REP administration. That the second strain was sensitive to INH at the concentratin of 0.01 microgram/ml suggested the availability of the combined use of INH in the chemotherapy of leprosy.</p>  a0001-5938