02632nas a2200529   4500000000100000008004100001260001600042653002500058653001300083653002400096653001800120653002200138653001700160653003100177653001800208653002000226653001600246653001100262653002000273653001200293653002400305653002500329653001800354653004500372653001300417653002500430653001300455653002700468653002500495100001600520700001400536700001400550700001500564700001200579700001400591700001600605700001200621700001600633700001700649700001500666245013600681856006100817300001100878490000800889520119100897022001402088       2000                            d  c2000 May 0110aAntigen Presentation10aAntigens10aAntigens, Bacterial10aAntigens, CD110aAntigens, Surface10aCD4 Antigens10aCD4-Positive T-Lymphocytes10aCell Membrane10aCells, Cultured10aGlycolipids10aHumans10aLectins, C-Type10aleprosy10aLipopolysaccharides10aMycobacterium leprae10aMycolic Acids10aNK Cell Lectin-Like Receptor Subfamily B10aPeptides10aProtein Biosynthesis10aProteins10aReceptors, Immunologic10aT-Lymphocyte Subsets1 aSieling P A1 aOchoa M T1 aJullien D1 aLeslie D S1 aSabet S1 aRosat J P1 aBurdick A E1 aRea T H1 aBrenner M B1 aPorcelli S A1 aModlin R L00aEvidence for human CD4+ T cells in the CD1-restricted repertoire: derivation of mycobacteria-reactive T cells from leprosy lesions.  uhttp://www.jimmunol.org/content/164/9/4790.full.pdf+html  a4790-60 v1643 a<p>Both the CD4-CD8- (double negative) and CD4-CD8+ T cell lineages have been shown to contain T cells which recognize microbial lipid and glycolipid Ags in the context of human CD1 molecules. To determine whether T cells expressing the CD4 coreceptor could recognize Ag in the context of CD1, we derived CD4+ T cell lines from the lesions of leprosy patients. We identified three CD4+ Mycobacterium leprae-reactive, CD1-restricted T cell lines: two CD1b restricted and one CD1c restricted. These T cell lines recognize mycobacterial Ags, one of which has not been previously described for CD1-restricted T cells. The response of CD4+ CD1-restricted T cells, unlike MHC class II-restricted T cells, was not inhibited by anti-CD4 mAb, suggesting that the CD4 coreceptor does not impact positive or negative selection of CD1-restricted T cells. The CD4+ CD1-restricted T cell lines produced IFN-gamma and GM-CSF, the Th1 pattern of cytokines required for cell-mediated immunity against intracellular pathogens, but no detectable IL-4. The existence of CD4+ CD1-restricted T cells that produce a Th1 cytokine pattern suggests a contributory role in immunity to mycobacterial infection.</p>  a0022-1767