01414nas a2200253   4500000000100000008004100001260001700042653002400059653001100083653001400094653001100108653002500119653002500144653003100169653002500200653000900225653001800234100001500252700001700267245010900284300000900393520074400402022001401146       1996                            d  c1996 Jan-Feb10aAntigens, Bacterial10aBiopsy10aGranuloma10aHumans10aImmunohistochemistry10aLeprosy, lepromatous10aMicroscopy, Immunoelectron10aMycobacterium leprae10aSkin10aSkin Diseases1 aMaslov A K1 aDiachina M N00a[The electron immunochemical detection of mycobacterial antigens in the granulomas of leprosy patients].  a84-73 a<p>M.leprae antigens (Ag) in skin granuloma macrophages from 10 untreated patients with active lepromatous leprosy were studied with the use of electron microscopic immunochemical methods. Peroxidase-conjugated antirabbit antibodies to M.leprae and M.avium were used. M.leprae Ag were found to be located in mycobacterial microcapsules, as well as in collagen fibers of the derma and the connective tissue of blood vessels with the signs of damaged collagen. In the latter case fixed immune complexes producing a damaging action on collagen fibers were probably detected together with free M.leprae Ag. The use of immunocytochemical methods may be rather valuable in the diagnosis of the indeterminate and tuberculoid forms of leprosy.</p>  a0372-9311