02496nas a2200349   4500000000100000008004100001260001300042653000900055653001200064653003000076653001100106653001900117653001300136653004100149653001100190653001200201653000900213653001600222653003000238653004600268653003000314100001100344700001300355700001300368700001500381700001200396245009000408300001000498490000700508520161700515022001402132       1996                            d  c1996 Jul10aAged10aAlleles10aDrug Resistance, Multiple10aFemale10aGene Frequency10agenotype10aHistocompatibility Antigens Class II10aHumans10aleprosy10aMale10aMiddle Aged10apolymerase chain reaction10aPolymorphism, Restriction Fragment Length10aWorld Health Organization1 aJoko S1 aNumaga J1 aMasuda K1 aNamisato M1 aMaeda H00a[HLA class II alleles and leprosy (Hansen's disease) classified by WHO-MDT criteria].  a121-70 v653 a<p>Human leukocyte antigens (HLA) class II alleles were analyzed in Japanese leprosy patients to ascertain whether immunogenetic differences exist among the forms of leprosy in classification of World Health Organization-recommended multidrug therapy (WHO-MDT). The subjects were 86 unrelated Japanese leprosy patients, including 62 multibacillary leprosy (MBL), 24 paucibacillary leprosy (PBL). Controls were 114 unrelated healthy subjects. Genotyping of HLA class II alleles was performed by using the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and PCR-restriction fragment length polymorphism (RFLP) methods. The frequencies of HLA-DRB1* 1501, * 1502 and DRB5* 0101,* 0102 and DQA1* 0102 and DQB1* 0602 were significantly increased in the whole patients (44.2%, 34.9%, 44.2%, 34.9%, 53.4% and 41.9%, respectively) as compared with the control subjects (14.0%, 21.1%, 14.0%, 21.1%, 27.2% and 13.2%, respectively). On the other hand, the frequencies of HLA-DRB1* 0405, * 0803, * 0901 and DQA1* 03 and DQB1* 0401 were significantly decreased in the whole patients (10.5%, 5.8%, 16.3%, 41.9% and 9.3%, respectively) as compared with the control subjects (29.8%, 17.5%, 30.7%, 78.1% and 29.8%, respectively). When MBL and PBL patients were compared, the frequencies of HLA-DRB1* 1501, DRB5* 0101 and DQB1* 0602 were significantly increased in the MBL patients (51.6%, 51.6% and 48.4%, respectively) as compared with the PBL patients (25.0%, respectively). Our results suggest that HLA-DRB1* 1501, DRB5* 0101 and DQB1* 0602 contribute to the susceptibility to the Japanese MBL.</p>  a0386-3980