02880nas a2200349   4500000000100000008004100001260001300042653002300055653002300078653002000101653001100121653002300132653001200155653002800167653002600195653001800221653002400239653002500263653002500288653001800313653002500331653002300356653002600379100001600405245012700421856004100548300001100589490000700600050001700607520189200624022001402516       1996                            d  c1996 Sep10aBacterial Proteins10aBacterial Vaccines10aCells, Cultured10aHumans10aImmunity, Cellular10aleprosy10aLeukocytes, Mononuclear10aLymphocyte Activation10aMycobacterium10aMycobacterium bovis10aMycobacterium leprae10aRecombinant Proteins10aT-Lymphocytes10aVaccines, Attenuated10aVaccines, Combined10aVaccines, Inactivated1 aMustafa A S00aRestoration of proliferative response to M. leprae antigens in lepromatous T cells against candidate antileprosy vaccines.  uhttp://ila.ilsl.br/pdfs/v64n3a02.pdf  a257-670 v64  aMUSTAFA 19963 a<p>Several studies conducted in the last decade suggest that Mycobacterium lepraereactive T cells exist in lepromatous patients, but their number may be too few to yield a detectable response in cell-mediated immunity (CMI) assays. Immunizations with candidate antileprosy vaccines and stimulation of T cells with M. leprae + interleukin-2 restore the M. leprae-induced CMI response in lepromatous leprosy patients. These immunizations and stimulation may enrich the pre-existing M. leprae-responsive T cells in lepromatous patients and, thereby, induce a detectable CMI response to M. leprae antigens upon repeat testing. To verify this proposition, we carried out a study in a group of 10 lepromatous leprosy patients. Peripheral blood mononuclear cells (PBMC) obtained from these patients were anergic to M. leprae antigens in proliferative assays, but they responded to the antigens of candidate antileprosy vaccines, i.e., M. bovis BCG, M. bovis BCG + M. leprae, and Mycobacterium w. The enrichment of M. leprae-responsive T cells was performed by establishing T-cell lines from the PBMC after in vitro stimulation with M. leprae, M. bovis BCG, M. bovis BCG + M. leprae, and Mycobacterium w. When tested for their proliferative responses, 1/10, 3/10, 6/10 and 2/10 T-cell lines established against M. leprae, M. bovis BCG, M. bovis BCG + M. leprae, and Mycobacterium w, respectively, responded to M. leprae. These results suggest that enrichment of pre-existing M. leprae-responsive T cells may contribute to the restoration of the T-cell response to M. leprae in some lepromatous patients. Four of the 10 M. leprae-induced T-cell lines proliferated in response to the 65 kDa, 36 kDa, 28 kDa, and 12 kDa recombinant antigens of M. leprae, suggesting that the nonresponsiveness of T cells in some lepromatous patients may be overcome by using recombinant antigens of M. leprae.</p>
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