01720nas a2200325   4500000000100000008004100001260001700042653001200059653001200071653001100083653002800094653002300122653001200145653001600157653000900173653001700182653001200199653001300211653001800224653000900242100001300251700001500264700001400279245011400293300001100407490000700418050003200425520092300457022001401380       1995                            d  c1995 Jul-Sep10aAnimals10aDapsone10aHumans10aImmunoenzyme Techniques10aLeprostatic Agents10aleprosy10aMacrophages10aMice10aNerve Tissue10aRabbits10aRifampin10aSchwann Cells10aSkin1 aSave M P1 aShetty V P1 aAntia N H00aIntracellular localization of dapsone and rifampicin in skin and nerve of multidrug treated leprosy patients.  a273-840 v67  aInfolep Library - available3 a<p>Intracellular localization of antileprosy drugs dapsone (DDS) and rifampicin (RFP) was carried out on skin and nerve lesions obtained from multidrug treated, multi (BL-LL)- and pauci (BT-TT) bacillary cases of leprosy using immunocytochemical techniques. Intracellular localization of the above drugs especially in macrophages and Schwann cells was aimed by using rabbit raised anti DDS and RFP polyclonal antibody in an indirect peroxidase assay. Our study records both intra and extracellular staining with anti DDS and RFP antibodies in the skin as well as nerve lesions of MB and PB cases treated with MDT. All the nerves under investigation had moderate to severe pathology and hence free diffusion of the drug could be attributed to the broken barrier. Basal lamina around the Schwann cell did not seem to form a barrier. It was also noted that the drug (metabolite) persisted over a long period of time).</p>  a0254-9395