01207nas a2200313 4500000000100000008004100001260001300042653001000055653001800065653001900083653001100102653001100113653000900124653001600133653002800149653002500177653002200202653003000224100001700254700001200271700001100283700001300294245008900307856004100396300000800437490000700445520042700452022001400879 1996 d c1996 Mar10aAdult10aBase Sequence10aDNA, Bacterial10aFemale10aHumans10aMale10aMiddle Aged10aMolecular Sequence Data10aMycobacterium leprae10aPeripheral nerves10apolymerase chain reaction1 aChemouilli P1 aWoods S1 aSaid G1 aCole S T00aDetection of Mycobacterium leprae in nerve lesions by the polymerase chain reaction. uhttp://ila.ilsl.br/pdfs/v64n1a01.pdf a1-50 v643 a

A simple procedure is described for the detection of Mycobacterium leprae by the polymerase chain reaction in nerve biopsies sectioned with a cryostat and then treated with proteinase K. All samples from lepromatous leprosy patients and the majority of samples from paucibacillary cases yielded positive results. This approach may be useful for differentiating between leprosy and other inflammatory neuropathies.

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