02205nas a2200241   4500000000100000008004100001260001300042653002600055653002400081653001100105653001900116653002100135653001200156653002500168100001400193700001400207245015200221856004100373300001000414490000700424520151800431022001401949       1996                            d  c1996 Mar10aAntibodies, Bacterial10aAntigens, Bacterial10aHumans10aImmunoblotting10aImmunoglobulin G10aleprosy10aMycobacterium leprae1 aKifayet A1 aHussain R00aIgG subclass recognition pattern in leprosy: recognition of M. leprae antigens by IgG1 and IgG3 antibodies is distinct across the disease spectrum.  uhttp://ila.ilsl.br/pdfs/v64n1a10.pdf  a69-780 v643 a<p>The recognition of Mycobacterium leprae antigens by IgG subclasses in patients with leprosy was investigated by electrophoresing M. leprae sonicate in SDS-polyacrylamide gel and immunoblotting analysis. Serum pools were used from leprosy patients with either lepromatous (LL/BL) or tuberculoid (BT/TT) disease. A serum pool from healthy controls (EC) was used to determine the baseline antibody activity. To adjust for quantitative differences in antibodies across the disease spectrum, the LL/BL serum pool was used at a 1:200 dilution; the BT/TT serum pool, at 1:20 dilution. Monoclonal antibodies specific for each of the IgG subclasses were used as probes, with anti-mouse IgG conjugated to alkaline phosphatase as the revealing probe. IgG1 antibodies bound to several discrete bands in the range of 10-70 kDa in LL/BL patients, while BT/TT patients showed a more diffuse pattern with the strongest IgG1 antibody binding in the region of 25-40 kDa. Recognition with IgG2 was restricted to a region between 25-36 kDa (which also stained strongly for carbohydrates) in both LL/BL and BT/TT patients. Binding with IgG3 antibodies was more restricted than IgG1 antibodies in LL/BL sera with strong recognition restricted to 25 and 28 kDa. BT/TT sera showed strong binding with IgG3 antibodies in the region of 25-32 as well as 5-7 kDa. IgG4 antibodies showed weak binding to a 28-kDa in lepromatous patients only. The differences in IgG subclass recognition patterns and their implications are discussed.</p>
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