02061nas a2200289   4500000000100000008004100001260001300042653002000055653001400075653001100089653002800100653001400128653001200142653004400154653000900198653001800207653002800225100001300253700001400266700001300280700001400293245013500307300001000442490000700452520129800459022001401757       1993                            d  c1993 Jan10aGene Expression10aGranuloma10aHumans10aImmunoenzyme Techniques10aIncidence10aleprosy10aReceptors, Antigen, T-Cell, gamma-delta10aSkin10aSkin Diseases10aTuberculosis, Cutaneous1 aFujita M1 aMiyachi Y1 aNakata K1 aImamura S00aGamma delta T-cell receptor-positive cells in human skin. I. Incidence and V-region gene expression in granulomatous skin lesions.  a46-500 v283 a<p><b>BACKGROUND: </b>There have been many reports that gamma delta T-cell receptor (TCR)+ cells respond to mycobacterial antigens in vitro, but there is little available information on human gamma delta TCR+ cells in clinical conditions.</p><p><b>OBJECTIVE: </b>Our purpose was to investigate the distribution and involvement of human gamma delta TCR+ cells in granulomatous skin lesions.</p><p><b>METHODS: </b>The incidence and V-region gene expression of human gamma delta TCR+ cells was examined in granulomatous skin diseases, including cutaneous tuberculosis and leprosy, by immunohistochemical procedures.</p><p><b>RESULTS: </b>gamma delta TCR+ cells in the dermis were increased in most patients with borderline lepromatous leprosy, and they were less frequently found in lepromatous leprosy and erythema nodosum leprosum. Other granulomatous skin lesions, including sarcoidosis, contained only a few gamma delta TCR+ cells. The gamma delta TCR+ cells that were found to be increased in this study were mostly delta TCS1-, BB3+, Ti gamma A+ (V delta 1-, V delta 2+, V gamma 9+).</p><p><b>CONCLUSION: </b>The gamma delta TCR+ cells in human granulomatous skin lesions may respond to some mycobacterial antigens, but they do not appear to be directly involved in granuloma formation.</p>  a0190-9622