02338nas a2200373   4500000000100000008004100001260000900042653002600051653002400077653001600101653002900117653002000146653001100166653004300177653001200220653002700232653001800259653002400277653002400301653002500325653002400350653003100374653002400405100001300429700001500442700001200457700001500469700001600484245015200500300001100652490000600663520128100669022001401950       1979                            d  c197910aAntibodies, Bacterial10aAntigens, Bacterial10aBCG Vaccine10aCorynebacterium pyogenes10aCross Reactions10aHumans10aImmunoelectrophoresis, Two-Dimensional10aleprosy10aListeria monocytogenes10aMycobacterium10aMycobacterium avium10aMycobacterium bovis10aMycobacterium leprae10aMycobacterium phlei10aMycobacterium tuberculosis10aNocardia asteroides1 aHarboe M1 aMshana R N1 aCloss O1 aKronvall G1 aAxelsen N H00aCross-reactions between mycobacteria. II. Crossed immunoelectrophoretic analysis of soluble antigens of BCG and comparison with other mycobacteria.  a115-240 v93 a<p>Cross-reactions between Mycobacterium bovis BCG and various other mycobacteria, Nocardia asteroides, Corynebacterium pyogenes and Listeria monocytogenes were studied by incorporating antibodies against these bacteria in the intermediate gel of a crossed immunoelectrophoretic system with BCG antigen and anti-BCG antibodies. In the BCG reference system forty-four distinct antigenic components were recorded, of which thiryt-three cross-reacted with Mycobacterium tuberculosis, twenty-five with M. avium, twenty-one with M. suvalii, eighteen with M. smegmatis, Fifteen with M. nonchromogenicum, twelve with M. phlei, eight with N. anteroides and two with C. pyogenes, whereas no cross-reaction was detected with L. monocytogenes. The value of the method for characterization of mycobacterial antigens is discussed. A taxonomic system based on this method appears particularly valuable for studies of non-cultivativable mycobacteria such as M. leprae. A majority of twenty-one patients with lepromatous leprosy had anti-BCG antibodies of restricted specificity, affecting only four or five BCG antigens, although one patient had twelve anti-BCG specificities. Most of these antibodies reacted with those BCG antigens that cross-react extensively with other mycobacteria.</p>  a0300-9475