01999nas a2200289   4500000000100000008004100001260001300042653003500055653001100090653001300101653001300114653001200127653002500139653004300164653004400207653000900251653002500260100001300285700001400298700001300312700001400325245015300339300001000492490000700502520118600509022001401695       1993                            d  c1993 Jan10aFluorescent Antibody Technique10aHumans10aKinetics10aLepromin10aleprosy10aMycobacterium leprae10aReceptors, Antigen, T-Cell, alpha-beta10aReceptors, Antigen, T-Cell, gamma-delta10aSkin10aT-Lymphocyte Subsets1 aFujita M1 aMiyachi Y1 aNakata K1 aImamura S00aAppearance of gamma delta T cell receptor-positive cells following alpha beta T cell receptor-positive cells in the lepromin reaction of human skin.  a39-440 v353 a<p>To elucidate the involvement of human gamma delta T cell receptor (TcR)+ cells in mycobacterial infection, we examined the kinetics of these cells in skin lesions of human lepromin reaction. The majority of CD3+ cells two days after induction of the lepromin reaction were alpha beta TcR+, while gamma delta TcR+ cells accounted for only 4.4 +/- 1.4% of the CD3+ cells. On day 21, the incidence of gamma delta TcR+ cells was greater (16.0 +/- 2.1%), although alpha beta TcR+ cells remained the predominant population. These kinetics of alpha beta TcR+ cells and gamma delta TcR+ cells contradict the 'early response, self-surveillance' hypothesis for gamma delta TcR+ cells in mice. Most of the gamma delta TcR+ cells in this study of the lepromin reaction were V delta 1- V delta 2+ V delta 9+, and some of them proliferated in the skin lesions, suggesting that gamma delta TcR+ cells in the lesions may respond to mycobacterial antigens and may play an active part in the lepromin reaction. However, these gamma delta TcR+ cells were not correlated with granuloma formation, the size of necrotic areas, mycobacterial content, or the incidence of CD4+ cells and CD8+ cells.</p>  a0165-2478