01327nas a2200289   4500000000100000008004100001260001300042653001100055653001600066653001900082653003000101653001100131653001200142653002500154653003000179653003100209653003200240653000900272100001400281700001200295700001600307245008500323300000900408490000600417520060000423022001401023       1993                            d  c1993 Feb10aBiopsy10aColorimetry10aDNA, Bacterial10aElectrophoresis, Agar Gel10aHumans10aleprosy10aMycobacterium leprae10apolymerase chain reaction10aReproducibility of Results10aSensitivity and Specificity10aSkin1 aVliet G M1 aWit M Y1 aKlatser P R00aA simple colorimetric assay for detection of amplified Mycobacterium leprae DNA.  a61-60 v73 a<p>A colorimetric assay for the detection of PCR-products is described. The assay is based on amplification of DNA in the presence of digoxigenin-dUTP. After immobilization of the PCR products to a microtitre plate, amplified DNA could be detected colorimetrically. The sensitivity of this colorimetric assay was equal to gel-analysis allowing the detection of 100 fg of template DNA. Here, we show that it can be used to detect Mycobacterium leprae DNA in biopsy specimens from leprosy patients. The simplicity and the low degree of variation make this assay an alternative to gel-analysis.</p>  a0890-8508