01472nas a2200301   4500000000100000008004100001260001300042653001200055653002600067653001500093653001900108653001100127653002500138653003200163653002500195653001400220653001400234653001300248653001500261100001500276700001500291700001600306245009700322300001000419490000700429520072000436022001401156       1993                            d  c1993 Mar10aAnimals10aAnti-Bacterial Agents10aArmadillos10aDrug Synergism10aHumans10aLactams, Macrocyclic10aMicrobial Sensitivity Tests10aMycobacterium leprae10aOfloxacin10aRifabutin10aRifampin10aRifamycins1 aDhople A M1 aIbanez M A1 aGardner G D00aIn vitro synergistic activity between ofloxacin and ansamycins against Mycobacterium leprae.  a384-60 v433 a<p>The antimicrobial effects of ofloxacin, alone and in combination with either rifampicin or rifabutin, were evaluated against M. leprae, using in vitro cell-free culture system. The minimum inhibitory concentration (MIC) of ofloxacin against M. leprae was 1.5 micrograms/ml, while MICs of rifampicin and rifabutin were 0.4 and 0.2 microgram/ml, respectively. Combination of 0.375 microgram/ml ofloxacin and 0.05 microgram/ml of rifabutin exhibited synergistic bactericidal activity while the effect of combination of 0.75 microgram/ml ofloxacin and 0.2 microgram/ml rifampicin was additive bactericidal. Thus, combination of ofloxacin and rifabutin deserves further attention in multi-drug therapy of leprosy.</p>  a0004-4172